Permanent fluorescent staining of nucleic acids in isolated cells.
نویسندگان
چکیده
منابع مشابه
Fluorescent Nucleic Acids
A new modular approach to the design and discovery of fluorophores and sensors built on a DNA backbone is described. Fluorescent designer nucleosides are synthesized into short oligomers; their close interactions results in highly efficient reporters with properties not seen in standard fluorophores. Applications in sensing of molecules, ions, and enzymes in water and in the vapor phase are des...
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Nucleic acids are typically detected in morphologically preserved fixed cells and tissues using in situ hybridization techniques. This review discusses a variety of established and more challenging fluorescence-based methods for the detection and tracking of DNA or RNA sequences in living cells. Over the past few years, various fluorescent in vivo labeling methods have been developed, and dedic...
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Silver staining was introduced more than a decade ago as a sensitive procedure to detect trace amounts of proteins in polyacrylamide gels (1). Since then, silver staining has been perfected and extended to the study of other biological molecules that have been separated by electrophoresis in a variety of supports (2,3). Silver staining follows generally one of two methods. One uses diamine or a...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1978
ISSN: 0022-1554,1551-5044
DOI: 10.1177/26.11.722051